主要功能
●对自然水体中的蓝藻、绿藻、硅/甲藻和隐藻自动分类(定性)
●自动测量水样中蓝藻、绿藻、硅/甲藻和隐藻的叶绿素 a 含量(定量)和总叶绿素 a 含量
●一杯自然水样,同时获得蓝藻、绿藻、硅/甲藻和隐藻的光合活性:
●光合效率和光合速率(相对电子传递速率)
●快速光曲线并进行拟合
●藻类的潜在最大光合效率
●藻类的光保护能力
●藻类耐受强光的能力
●用户可做自己的参考光谱
测量参数
Fo, Fm, F, Fm', Fv/Fm, Y(II) 即 ΔF/Fm', ETR, a, Ik, Pm, PAR 和叶绿素含量等
应用领域
●主要用于水生生物学、水域生态学、海洋学、湖沼学、水质预警、微藻生理学、微藻抗逆性等领域,对于了解自然水样中藻类种群的动态变化、水华预警、野外水体中光合作用的时空变化、校正初级生产力的计算等有较大帮助。
●特别适于浮游植物动力学研究和有害水华的早期预警。
主要技术参数
●主控单元:金属外壳,包含所有的光电元件及样品测量室
●测量光: LED,440 nm,480 nm,540 nm,590 nm 和 625 nm,5 波长脉冲调制测量光,2 档强度设置,8 档调制频率设置, 3 档测量光自动高频设置
●光化光:板载多波长 LED 阵列,440 nm,480 nm,540 nm,590 nm,625 nm 和 420-640 nm(白光),提供持续光化光,最高可达 1400 μmol m-2 s-1,快速动力学闪光高达 7000 μmol m-2 s-1,饱和脉冲最高可达 5000 μmol m-2 s-1
●远红光:725 nm LED
●信号检测:基于 H-10720 光电传感器模块的光电倍增检测器
●标准检测过滤器:> 650 nm 长通滤光片
●高分辨率的光电倍增管,叶绿素浓度检测限低至 0.1μg/L,适用野外采集的藻浓度很稀的样品
升级的技术特点
●可提供 5 种波长的脉冲调制测量光和光化光
●板载 LED 阵列芯片技术
●可实时进行四种藻的分类
●可进行标准 PAM 测量及不同波长强光化光诱导的毫秒级荧光上升动力学分析
●可测定光系统 II 功能性捕光截面积
●内置自动测量程序,易于操作
PHYTO-PAM-II vs PHYTO-PAM
便携式 PHYTO-PAM-II | PHYTO-PAM |
5 种不同波长的测量光用于生物体内不同类型的天线色素荧光的激发 | 4 种不同波长的光 |
4 种色素类型的在线分类 | 3 种色素类型的在线分类 |
6 种波长的光化光 | 1 种波长的光化光 |
可以分别测量绿藻,蓝藻,硅/甲藻以及含有藻红蛋白的有机体,如隐藻不同波长下 PSII 的活性 | 无该功能 |
紧凑型设计 | 需要组装 |
增加了快速动力学操作模式,可通过强光化光脉冲,测量不同波长的 O- I1 荧光上升动力学曲线 | 无该功能 |
通过测定不同光质和光合生物色素复合体,获得光系统 II 功能性捕光截面积的信息即 σPSII | 无该功能 |
通过 FluoRed 荧光标准将参考光谱校准标准化 | 无该功能 |
获得的参考光谱可在不同设备及用户间互换使用 | 参考光谱不能互换 |
选购指南
一、实验室测量基本款
系统组成:实验室版主机,激发检测单元,悬浮液的光学单元,球形光量子传感器,工作台,软件等
注意:新版 PHYTO-PAM-II 必须配置光量子传感器,校准光强后参考光谱可在不同仪器间通用
实验室测量基本款 |
二、野外便携紧凑款
系统组成:紧凑型主机,球形光量子传感器,软件等
注意:新版 PHYTO-PAM-II 必须配置光量子传感器,校准光强后参考光谱可在不同仪器间通用
便携式紧凑款 |
五种测量光通道 | 区分四种藻类 | 通用型参考光谱 |
测量慢速动力学曲线 淬灭分析,暗弛豫分析 | 测量快速光曲线 光响应曲线 | 测量快速动力学曲线 分析PSII功能性捕光截面 |
三、其他可选附件
1,WATER-S:搅拌器(推荐选配),利用内置电池驱动的马达带动搅拌棒旋转,对样品杯里的悬浮液进行搅拌。需配置搅拌棒 WATER-R。
2,WATER-R:搅拌棒(标配10根)
产地:德国WALZ
参考文献
数据来源:光合作用文献 Endnote 数据库,更新至 2021 年 1 月,文献数量超过 10000 篇
原始数据来源:Google Scholar
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