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“AgriPheno论坛”（8月20日）：Applications of Automated DNA Mass Spectrometry in Agribiotech

报告题目：Applications of Automated DNA Mass Spectrometry in Agribiotech
报 告 人：Dr. Darryl Irwin, Director–Applications & Technology, Asia Pacific, Sequenom
报告时间：2014年8月20日14:30
报告地点：上海市浦东新区沔北路185号孙桥现代农业园C9-1 AgriPheno平台2楼会议室
联 系 人：魏渊源(yywei@zealquest.com)
报告摘要：

Sequenom’s automated DNA analysis platform has recently been broadly used in a variety of species important in agriculture and livestock. A single MassARRAY system can be used for the analysis of DNA sequence variations like SNPs, gene expression, DNA methylation, copy number variation and certain DNA sequencing applications. The sensitivity is 100 to 1,000 times higher than real time PCR and the quantification is an order of magnitude better than real time PCR. But perhaps the strongest feature of the technology is the ease and flexibility with which new problem-specific applications can be developed and validated. This is especially important for agribiotech where so many diverse species are of interest. For example, with commercial livestock and crops, panels of 10s to 100s of SNPs can be rapidly developed that are extremely powerful for individual identification, breeding, and feedlot/nutrient management. The increased use of high content discovery technologies such as 2nd Generation Sequencing and microarrays in agricultural research has resulted in a clear need for validation of discovered markers. With plants, ploidies higher than 2, mean that essentially every locus must be treated as a potential copy number variant, and mass spectrometry offers a facile way to quantify such variants in a cost-effective multiplexed manner. DNA mass spectrometry also shows great promise in the automated identification and typing of micro-organisms, and thus might be very useful in the study of a wide variety of plant and animal infectious diseases. This seminar will review the basics of the technology and then illustrate a number of current case studies of the above applications.

关于“AgriPheno论坛”
“AgriPheno论坛”是由高通量植物基因型-表型-育种平台AgriPheno推出的一个学术交流活动平台，旨在促进国内外相关学者、育种家、发明家和应用科学家与AgriPheno平台和上海的农业科技人员之间的交流、互动。该论坛不定期于AgriPheno平台（上海市浦东新区沔北路185号孙桥现代农业园C9-1）举行，每次会提前通过网站、微信等渠道发布通知。我们诚挚邀请相关学者、育种家、发明家和应用科学家在访问AgriPheno平台时通过讲座报告的形式参与该论坛；我们热诚欢迎相关科研人员和研究生参与论坛的交流活动。