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叶绿素荧光成像系统——M-IMAGING-PAM
日期:2025-04-08 11:57:45

功能强大、操作最简便、发表文献超多的叶绿素荧光成像系统

突变株快速筛选的强大工具


MAXI版本.jpgMINI.pngMICROSCOPY版本.jpg

MAXI版本,成像面积11×15   cm)

蓝光版,450 nm,测叶片和真核藻类

红光版,620 nm,测蓝藻和真核藻类

可测量96孔板,平板,384点藻斑

MINI版本,成像面积2.4×3.2 cm

蓝光版,460 nm,测量叶片和真核藻类

红光版,620 nm,测量叶片和蓝藻

GFP版,480 nm,测量绿色荧光蛋白

MICROSCOPY版本

蓝光版:470 nm

红光版:625nm

RGB版:RGB光源

M-IMAGING-PAM是一套主机可分别接多个成像单元的叶绿素荧光成像系统,如MAXI成像单元,MINI成像单元以及搭载显微镜的MICROSCOPY成像单元。不同的成像单元适用于不同的应用场景,MAXI成像单元主要在实验室内使用,MINI成像单元可以带到野外现场使用,MICROSCOPY成像单元可以用于组织或藻类单细胞测量。

每个成像单元有蓝光和红光两个类型的光源区分,它们的区别在于蓝光版主要用于高等植物和真核藻类的测量,而红光版可以用于蓝藻或蓝藻共生的生物结皮,地衣测量。除此之外,MINI成像单元还有一个单独的GFP版本,可以用于检测遗传标签表达的GFP荧光蛋白。MICROSCOPY成像单元的RGB光源可以用于藻类种群信号解卷积,进行单细胞藻类分类。

M-IMAGING-PAM充分考虑了叶绿素荧光成像系统设计的基本原则:测量光强度足够低的情况下最大限度的保证了有效成像面积内的光场均匀性(最大差异小于2-7%)。广场均匀的基础上,M-IMAGING-PAM还充分考虑了绝大部分研究的需求,光化光和饱和脉冲强度均可满足所有物种测量的基本要求。

M-IMAGING-PAM叶绿素荧光成像系统于2003年问世。时至今日,累计发表文献4000多篇,光合作用文献数据库收录2283篇,是发表文献最多的PAM型号。近五年,每年都有超过200篇文献发表。发文质量高,其中不乏Nature,Molecular Plant,Nature Plants,Nature Communications,The Plant Cell,PNAS,New Phytologist,Plant Physiology,The Plant Journal等植物学领域的专业高分杂志文章(详见附录)。

M-IMAGING-PAM是叶绿素荧光成像系统的典范,不是所有的叶绿素荧光成像系统都叫M-IMAGING-PAM。


主要功能

 

成像参数

Fo, Fm, F, Ft, Fm', Fv/Fm, Y(II), qL, qP, qN, NPQ, Y(NPQ), Y(NO), ETR, Abs, NIR, Red, Inh等

 

应用领域

  

扩展功能

IMAGIING-PAM与GFS-3000联用

IMAGIING-PAM与GFS-3000.jpg

模式一:MAXI-探头与GFS-3000联用,在10 cm x 13 cm的面积上同步测量气体交换与荧光成像。


模式二:MINI-探头与GFS-3000联用,在2 cm x 3.2 cm的面积上同步测量气体交换与荧光成像。


模式三:MINI-探头与GFS-3000和拟南芥叶室联用,实现拟南芥整株的同步测量(气体交换与荧光成像)。


联用1.jpg联用2.jpg



Phenoplate:MAXI-IMAGIING-PAM与热循环仪联用

Phenoplate方法集成了Maxi-Imaging-PAM和热循环仪,可在叶绿素a荧光测量之前、期间和之后对温度进行快速动态控制。它可以在动态控制的热环境中同时评估多达384个样品的光系统II效率(Y(II))和非光化学淬灭(NPQ)。在本文中,我们展示了如何利用这一简单的系统来详细描述光合作用&光强&温度之间在电子转移率(ETR)和非光化学淬灭(NPQ)方面的关系。

热循环仪联用.jpg热循环仪联用2.jpg

 

应用案例

突变株的快速筛选

MAXI-IMAGING-PAM特别适合对幼苗、愈伤组织、微藻等进行突变株的快速筛选,适合于与光合突变株、抗逆(抗旱、抗盐、抗病等)突变株、产油/氢突变株等的快速筛选。

突变株的快速筛选1.jpg突变株的快速筛选2.jpg
                            突变株筛选案例:国内某客户使用384藻斑点筛选的衣藻突变株(左:Fo成像;右:Fv/Fm成像)


环境对光合生物的影响

高温,干旱,盐分等环境条件会对光合生物产生非生物胁迫,影响光合作用正常进行。Fv/Fm作为光系统II最大光化学转化效率常被用来指示胁迫对光合生物影响的程度,具有非常广泛的应用。

环境对光合生物.jpg

环境对光合生物的影响案例:全球变暖对珊瑚共生虫黄藻光合能力的影响。

 

光合生物对环境的自我适应与调节

自然界中的植物通常都能很好地应对环境变化带来的伤害,因为它们已经进化出了敏锐的应对机制。非光化学淬灭可以将过剩的激发能耗散为热量在其中起到了非常重要的作用。NPQ作为表征非光化学淬灭的荧光参数,在研究光下和暗弛豫阶段的能量分配中被广泛使用,具有非常重要的意义。

自我适应与调节1.jpg自我适应与调节2.jpg

                                      光合生物对环境的自我适应与调节案例:拟南芥短链脱氢酶-还原酶突变体光保护能力研究

 

产地:德国WALZ

 

高分文献目录

数据来源:光合作用文献Endnote数据库

原始数据来源:Google Scholar

 

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