主要功能
成像功能:对 Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等至少 17 种参数进行成像分析。测定调节性能量耗散 Y(NPQ),反映植物光保护能力,测定非调节性能量耗散Y(NO),反映植物光损伤程度。
程序测量功能:可程序测量荧光诱导曲线、快速光曲线和暗弛豫,也可手动测量;在测量过程中能自动分析所有荧光参数的变化趋势
AOI 功能:可在测量前或测量后任意选择感兴趣的区域(AOI),程序将自动对选择的 AOI 的数据进行变化趋势分析,并在报告文件中显示相关 AOI 的数据。所有报告文件中显示的数据都可导出到 EXCEL 文件中。
成像异质性分析功能:对任意参数任意时间的成像,可在图像上任意选取两点,软件自动对两点间的数据进行横向异质性分析,并可导出到 EXCEL 文件中。
成像数据范围分析功能:对任意参数任意时间的成像,可分析任意两个荧光数值之间有多少个像素点,多少面积(cm2)。
突变株筛选功能:可跟据成像结果快速筛选光合、产氢/油、抗逆(抗盐、抗旱、抗病等)等突变株。
微藻毒理研究功能:可同时测量 96 个微藻样品(对照和处理组)的光合活性,软件自动给出处理组样品相对于对照组的光合抑制百分比。
吸光系数测量功能:快速测量叶片的吸光系数。吸光系数测量光源: 16 个红光(650 nm)和 16 个近红外(780 nm)LED,用于测量植物叶片或藻类样品 PAR 吸光系数。
光强异质性:测量区域光强异质性≤±2%
测量参数
Ft、Fo、Fm、Fv/Fm、F、Fm’、Y(II)、Y(NO)、Y(NPQ)、NPQ、qN、qP、qL、ETR、Abs.、NIR、Red 等。
应用领域
光合作用研究:可以在完全相同的条件下同时对大量样品进行成像
植物病理学:病斑部位(包括肉眼不可见时)成像以及病斑扩散的时空动力学
植物胁迫生 理学:肉眼不可见胁迫损伤的早期检测
遗传育种:出苗后大规模快速筛选高光合/抗旱/抗热/抗冻/抗病等植株
突变株筛选:快速筛选模式植物的光合突变株、抗逆突变株、产氢微藻突变株等
微藻毒理学:不同毒物浓度多个重复的样品一次测完,软件自动计算抑制比率
分子生物学:宏观水平上检测样品的绿色荧光蛋白(GFP)荧光
其它多种扩展研究
选购指南
一、荧光成像 MAXI 版
系统组成:通用型主机,LED 供电单元, LED 光源,CCD,数据线,软件等。
注意:高等植物或真核藻类测量时选择蓝光光源(推荐),蓝藻测量请选择红光光源。
荧光成像 MAXI 版本 |
二、荧光成像 MINI 版
系统组成:通用型主机(下图中未显示),LED 光源(如下图),CCD,数据线,软件等。
注意:高等植物或真核藻类测量时选择蓝光光源(推荐),蓝藻测量请选择红光光源。
荧光成像 MINI 版 |
三、荧光成像 MICROSCOPY 版
系统组成:通用型主机,特制显微镜,CCD,数据线,软件等。
荧光成像 MICROSCOPY 版 |
葡萄叶片 Y(NPQ) 成像 | 葡萄叶片 PS/50 成像结果 | MINI 版本成像结果 |
| | |
显微版本成像结果 | 软件成像界面 | 慢速诱导动力学曲线 |
产地:德国 WALZ
参考文献
数据来源:光合作用文献 Endnote 数据库,更新至 2021 年 1 月,文献数量超过 10000 篇
原始数据来源:Google Scholar
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