主要功能
本系统是全球检出限和灵敏度很高的乙烯监测系统，主要用于植物研究相关的乙烯气体监测，如种子发芽、植物生长发育、开花生理、植物器官衰老、基因表达、植物病原体相互作用、植物激素间相互作用、蔬果收货后保藏、植物抗逆性研究（干旱、高温、重金属）等。
其中乙烯气体检测仪 ETD-300 采用先进的激光技术（光声学原理），即样品乙烯在光声腔吸收激光后释放热使光声腔内部产生压力，随激光频率增减形成能被微型麦克风检测到的压力差，而乙烯浓度越高压力差越大，从而据声波强度差可实时快速测量乙烯气体（C2H4）绝对浓度；阀门控制箱 VC-6 完全自动化和电脑控制，接一个即可以使单个气体检测仪实现6个样品的自动切换测量，单个乙烯气体检测仪可以接一个或多个阀门控制箱；烃分解器 CAT-1 则利用铂金颗粒催化烃氧化分解为水蒸气和 CO2，为系统提供无烃干扰的样品空气。
测量参数
测量参数：乙烯浓度（ppbv）、气体流速（l/h）、背景值、模拟输入（V）
计算参数：乙烯产量（nl/h）
连续流动测定（左）和积累测定（右）的乙烯监测数据图
应用领域
用于环境、医学、农业、工业、生态、生物等监测领域。特别适合植物生理、发育研究的超灵敏乙烯测量。
主要技术参数
参数 | 乙烯气体检测仪 ETD-300 | 阀门控制箱 VC-6 | 烃分解器 CAT-1 | |
测量范围 | 0-2 ppm / 0-100 ppm（可调） | / | / | |
检出限 | 0.3 ppbv | / | / | |
噪音(2σ) | 0.3 ppbv | / | / | |
精度 | <1% 或 0.3 ppbv | 0.2% FS | / | |
稳定性 | <1% 超过 24 小时 | / | / | |
零点漂移 | +/-1 ppbv | / | / | |
测量时间 | 7-9 s | / | / | |
响应时间 | 30 s （当流量为1 l/h时） | 300 ms | / | |
流量 | 0.25-5 l/h | 0.25-5 l/h | 0-30 l/h | |
校准 | 使用标准混合气，每年一次 | / | / | |
通道数量 | / | 6（可增至 12, 18 等） | / | |
测量模式 | / | 连续测量，积累测量 | / | |
气体供应压力 | / | 0.5-5 Bar | / | |
过压阀 | / | 在 5 Bar 时打开 | / | |
滤膜类型 | / | 去除粒径 >7μm 的微粒 | / | |
最大稀释浓度 | / | / | 100 ppm | |
输出浓度 | / | / | < 100 pptv | |
压力 | / | / | 0-6 atm | |
活性催化剂 | / | / | Pt/SiO2 | |
催化温度 | / | / | 150–250 ℃ | |
预热时间 | 30 min | / | < 10 min | |
尺寸 | 42x45x14 cm (48.3cm 3U 机架) | 30x45x10 cm (48.3cm 2U机架) | 33x24x14 cm （48.3 cm 3U 半机架） | |
工作温度/湿度 | 10-28 ℃ / 0-95 % RH | 5-40 ℃ / 0-95 % RH | 5-40 ℃ / 0-95 % RH | |
电源要求 | 90-264 VAC，47-63 Hz | 90-264 VAC，47-63 Hz | 90-264 VAC，47-63 Hz | |
功耗 | <150 W | <20 W | 85 W | |
进气接口 | 接外径 1/8'' 软管的快速接头 | 接外径 1/8'' 软管的快速接头 | 接外径 1/8'' 软管的快速接头 | |
模拟输入 | 0-5 V | / | / | |
数据输出 | USB，CSV 格式 | USB，CSV 格式 | / | |
显示 | 触摸屏 | LED 指示灯 | / |
选购指南：
6通道监测系统组成如下：
乙烯气体检测仪ETD-300 + 阀门控制箱VC-6 + 烃分解器CAT-1
注：系统中 3 个仪器都可以单独使用
可酌情选择单通道系统：乙烯气体检测仪 ETD-300+ 烃分解器 CAT-1。
产地：荷兰Sensor Sense 
应用举例
1.1 乙烯测定在高温胁迫研究中的应用举例
实验内容简介：以生长 3 周的拟南芥野生型 Col-0，突变体 NahG 和 opr3 植株为材料，研究了其高温胁迫下的乙烯释放。其中，野生型 Col-0 高温胁迫（38℃）下，电导率（电解质渗透率）、水杨酸和茉莉酸含量和乙烯释放增加；突变体 NahG 和 opr3 高温胁迫（38℃）下电导率、茉莉酸和乙烯释放也增加，但都低于野生型 Col-0，而高温胁迫后恢复阶段（水中 22℃）电导率明显高于 Col-0。研究结果表明：高温胁迫下，乙烯迅速产生，其生产受到茉莉酸和水杨酸的调控。总的来说，茉莉酸与水杨酸协同调节植物对高温胁迫的耐受，而乙烯主要加快细胞死亡；突变体 NahG 和 opr3 比野生型 Col-0 的耐热性差，细胞死亡多。
图1 高温处理下拟南芥植株的水杨酸（a）、电导率（b、c）和乙烯释放（d、e）
WT：拟南芥野生型；突变株opr3 ；突变株NahG以及培养基agar
Clarke, S.M., et al., Jasmonates act with salicyli c acid to confer basal thermotolerance in Arabidopsis thaliana. New Phytologist, 2009. 182(1): p. 175-187.
1.2 乙烯测定在营养缺乏（Mg）胁迫研究中的应用举例
实验内容简介：以生长5周的水培拟南芥 Col-0 植株为材料，研究了其缺镁胁迫下的乙烯释放。缺镁处理后乙烯生物合成酶基因（例如 At5g43450、At1g06620 和At2g25450）的表达水平明显上升，样品乙烯释放是对照组的两倍多，叶片中抗坏血酸 ASC 和谷胱甘肽 GSH 的氧化态比例增加。研究结果表明：植物应答缺镁胁迫存在一些独特的信号通路，且与植物激素有关，而乙烯在应答缺镁过程中发挥了关键作用；缺镁还同步增强了植物抗氧化酶活性。
表 1 镁元素缺乏处理第 8 天拟南芥新成熟叶片和根系的生理参数
DHA：ASC，氧化态脱氢抗坏血酸：抗坏血酸；GSSG : GSH，氧化型谷胱甘肽：谷胱甘肽；Ctrl，镁元素充足的植株；-Mg，镁元素缺乏的植株
Hermans, C., et al., Systems analysis of the responses to long-term magnesium deficiency and restoration in Arabidopsis thaliana. New Phytologist, 2010. 187(1): p. 132-144.
1.3 乙烯测定在病菌感染研究中的应用举例
实验内容简介：以品种为 Money Maker 和 Daniela 的成熟番茄果实为材料，研究了其感染番茄灰霉病菌株 VTF1 的乙烯释放。灰霉病菌可以在体外产生乙烯，其乙烯释放与其说与分生孢子萌发相关，不如说与菌丝生长更相关，且分生孢子浓度越大真菌的乙烯释放越多。感染灰霉病的两种番茄的乙烯释放规律与灰霉病菌类似；但释放量是其 100 倍。结合受感染番茄的细胞学参数，研究结果表明：番茄-真菌系统的乙烯释放不是由番茄灰霉病菌引起的，虽说与其内部的真菌生长速率十分同步。
图 2 真菌（160 μl 悬浮液）的乙烯产量
● 1.5*108 灰霉病菌分生孢子 ml-1 ▲ 2*107 灰霉病菌分生孢子 ml-1 ■ 2*105 灰霉病菌分生孢子 ml-1
图3 模拟感染和不同浓度番茄灰霉病菌感染的两种番茄的乙烯释放
A.番茄品种 Money Maker；B.番茄品种 Daniela；
○ 模拟番茄灰霉病菌感染 ● 1.5*108 灰霉病菌分生孢子 ml-1 ▲ 2*107 灰霉病菌分生孢子 ml-1 ■ 2*105 灰霉病菌分生孢子 ml-1
Cristescu, S.M., et al., Ethylene Production by Botrytis cinerea In Vitro and in Tomatoes. Applied and Environmental Microbiology, 2002. 68 (11): p. 5342-5350.
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